《植物生理学报》 2011, 47(6): 575-580
通信作者:曾英;E-mail: biochem@mail.kib.ac.cn;Tel: 0871-5223210
摘 要:
药用植物淫羊藿富含异戊烯类黄酮, LC-MS定量分析表明, 3~4月份粗毛淫羊藿叶片处于变色期时淫羊藿苷含量最 高。通过简并引物和RACE-PCR从粗毛淫羊藿叶片获得异戊烯转移酶同源基因(命名为EaPT1)。序列分析表明, EaPT1与 维生素E合成相关的尿黑酸异戊烯转移酶位于同一进化枝, 靠近类黄酮异戊烯转移酶。RT-PCR结果显示, EaPT1在叶片和 幼茎中表达显著。关键词:淫羊藿苷; 异戊烯转移酶; 异戊烯类黄酮; 粗毛淫羊藿
收稿:2011-01-06 修定:2011-01-06
资助:“973”计划(2007CB108802)和植物化学与西部植物资源 持续利用国家重点实验室自主课题(P2008-ZZ16和P2009- ZZ02)。
Corresponding author: ZENG Ying; E-mail: biochem@mail.kib.ac.cn; Tel: 0871-5223210
Abstract:
The traditional Chinese medicinal plant, Epimedium acuminatum, is remarkably rich in pharmacologically active prenylflavonoids. LC-MS analysis indicated that the leaves of Epimedium acuminatum had the highest icariin content in the color phase (from March to April). A prenyltransferase-like gene (named EaPT1) was cloned from those leaves with degenerate primers and subsequent rapid amplification of cDNA ends (RACE). The deduced protein sequence of EaPT1 grouped together with homogentisate phytyltransferases relevant to vitamin E biosynthesis and closely related to the flavonoid prenyltransferases. The EaPT1 transcripts significantly accumulated in leaves and young stems by RT-PCR.Key words: icariin; prenyltransferase; prenylflavonoid; Epimedium acuminatum
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